Paranoid
Posted at 07:11 PM on August 23, 2009.
For organic chem purposes...
Formal Report
Abstract
The experiment was performed to separate the colored
components of siling labuyo and malunggay leaves using column chromatography
and thin layer chromatography. Column chromatography was used to extract the
colored pigments of siling labuyo (Capisicum
frutescens) and malunggay leaves.
Siling labuyo has yellow, orage, and pale yellow pigments. While malunggay has
yellow, dark green, light green, and light yellow. Upon getting the colored pigments,
it was spotted to the thin layer chromatography (TLC) plate to determine the
purity of the components as well as to get the Retention factor (Rf) values of
the colored pigments. The results showed
that the yellow pigment is the most polar and the light yellow pigment is the
least polar, having an Rf value of 0.52 and 0.17 respectively.
Introduction
Chromatography is defined as any of various techniques for the qualitative or quantitative separation of the components of mixtures of compounds; all characterised by the use of a mobile phase (gas or liquid) moving relative to a stationary phase (liquid or solid). It is also a separation technique used for identifying compounds. Two types of chromatography were used in this experiment; column chromatography and thin layer chromatography. Such chromatographic methods were used because the experiment dealt with two phases, solid and liquid. The objectives of the experiment are: to be able to separate the colored pigments of siling labuyo and malunggay leaves using column chromatography, to determine the purity of the components using the thin layer chromatography, and to measure the Rf values of the colored components.
Experimental
Column
Chromatography
Red siling labuyo and malunggay leaves were grinded
separetly to extract the colored pigments. Upon grinding, it was eluted by
adding DCM-hexane (1:1) to siling labuyo and hexane:acetone (7:3) to malunggay
leaves. While waiting for the extractions, the other members of the group
already prepared the set-up for column chromatography. To create the set-up, they
get a Pasteur pipette, plugged small piece cotton at the narrow end of it,
filled it with silica gel up to the indented part of the Pasteur pipette, and
suspend it in an iron stand. They made
two set-ups, one for each extract. After getting the extractions, 0.5 ml of each crude extract was dropped on
top of the column and 3 ml of each eluents was added to separate the colored
components; hexane: acetone (7:3), DCM-he
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